人椎骨间充质干细胞
分离自人椎体。人椎骨间充质干细胞（HVMSC） 在第一代（P1）时进行冻存，并以冷冻状态交付。每瓶含有 >5 × 105 个细胞，总体积为 1 mL。
Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC have the capability for renewal and differentiation into various lineages of mesenchymal tissues. With their renewal capability, MSC have the potential to be transplanted into an injured site or seeded on a biomimetic scaffold to generate appropriate tissue constructs.
HVMSC from ScienCell Research Laboratories are isolated from human vertebral body. HVMSC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HVMSC are characterized by immunofluorescence with antibodies specific to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and Alizarin Red staining after osteogenic differentiation. HVMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HVMSC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing HVMSC in vitro.
间充质干细胞（MSC）是一类研究较为深入的成体干细胞，具有分化为脂肪细胞、软骨细胞、成骨细胞、肌腱细胞和肌肉细胞等成熟细胞的潜能。由于 MSC 具有较强的发育可塑性及多向分化能力，其在再生医学领域的潜在应用受到广泛关注。在无血清培养条件下添加转化生长因子，可诱导 MSC 向软骨细胞分化；而在含血清培养基中添加抗坏血酸和地塞米松，则可诱导 MSC 向成骨细胞分化。MSC 具有自我更新能力，并能够向多种间充质组织细胞谱系分化。因此，MSC 不仅可用于移植至组织损伤部位，还可接种于仿生支架上，用于构建适宜的组织。
ScienCell Research Laboratories 的 HVMSC 分离自人椎体，冻存于第一代（P1），并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。HVMSC 通过免疫荧光法，采用 CD73 和/或 CD90 特异性抗体进行表征；经成脂诱导分化后，通过油红 O 染色进行鉴定；经成骨诱导分化后，则通过茜素红染色进行鉴定。HVMSC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 推荐的培养条件下，HVMSC 可进一步扩增至少 15 个群体倍增。
推荐培养基
建议使用间充质干细胞培养基（MSCM，产品编号 7501）进行 HVMSC 的体外培养。
| 目录编号 | 7550 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HVMSC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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