人羊膜间充质基质细胞
人羊膜间充质基质细胞（HAMSC）分离自人羊膜。HAMSC在第1代培养时进行冻存，并以冷冻状态运输。每支冻存管含有>5 × 105个细胞，体积为1 ml。
Multipotent mesenchymal stromal cells, also known as mesenchymal stem cells (MSC), are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC have the capability for renewal and differentiation into various lineages of mesenchymal tissues. MSC derived from the amnion and chorion express CD271, possess characteristics of cardiomyocytes, and have various antiangiogenic and anti-inflammatory proteins.
HAMSC from ScienCell Research Laboratories are isolated from human amniotic membrane. HAMSC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HAMSC are characterized by immunofluorescence with antibodies specific to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and Alizarin Red staining after osteogenic differentiation. HAMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAMSC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing HAMSC in vitro.
多能性间充质基质细胞，又称间充质干细胞（MSC），是一类特征明确的成体干细胞。它们具有分化为成熟细胞的潜能，可形成脂肪、软骨、骨、肌腱和肌肉组织。由于具有这些特性以及发育可塑性，MSC在组织再生领域具有巨大的应用潜力，可用于替代受损组织，因此受到广泛关注。在无血清培养条件下，在转化生长因子的存在下培养MSC可诱导其分化为软骨细胞。相反，在含血清并添加抗坏血酸和地塞米松的培养条件下，MSC可分化为成骨细胞。MSC具有自我更新能力，并可分化为多种间充质组织谱系。来源于羊膜和绒毛膜的MSC表达CD271，具有心肌细胞的特征，并含有多种抗血管生成和抗炎蛋白。
ScienCell Research Laboratories提供的HAMSC分离自人羊膜。HAMSC在第1代培养时进行冻存，并以冷冻状态运输。每支冻存管含有>5 × 105个细胞，体积为1 ml。HAMSC采用针对CD73和/或CD90的特异性抗体进行免疫荧光鉴定，并分别在成脂分化和成骨分化后进行油红O染色和茜素红染色鉴定。HAMSC经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。根据ScienCell Research Laboratories提供的培养条件，HAMSC可保证进一步扩增15个群体倍增。
推荐培养基
建议使用间充质干细胞培养基（MSCM，产品编号7501）对HAMSC进行体外培养。
| 目录编号 | 7140 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HAMSC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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