人肺间充质干细胞

Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine. Fetal lung has been identified as a rich source of MSC and studies have shown that MSC can differentiate into neural cells, in addition to their mesenchymal differentiation potential. MSC have also been shown to enhance the engraftment of human umbilical cord blood-derived CD34 hematopoietic cells in immunodeficient mice.

HPMSC from ScienCell Research Laboratories are isolated from human lung tissue. HPMSC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HPMSC are characterized by immunofluorescence with antibodies specific to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and Alizarin Red staining after osteogenic differentiation. HPMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPMSC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing HPMSC in vitro.

间充质干细胞（MSC）是一类特征明确的成体干细胞。MSC具有分化为成熟细胞的潜能，可形成脂肪、软骨、骨、肌腱和肌肉组织。由于具有这些特性以及发育可塑性，MSC在再生医学领域具有巨大的应用潜力，因此受到广泛关注。胎肺已被确定为MSC的丰富来源，研究表明，除具有向间充质组织分化的潜能外，MSC还可分化为神经细胞。研究还表明，MSC能够促进人脐带血来源的CD34⁺造血细胞在免疫缺陷小鼠中的植入。

ScienCell Research Laboratories提供的HPMSC分离自人肺组织。HPMSC在第1代培养时进行冻存，并以冷冻状态运输。每支冻存管含有>5 × 10⁵个细胞，体积为1 ml。HPMSC采用针对CD73和/或CD90的特异性抗体进行免疫荧光鉴定，并分别在成脂分化和成骨分化后进行油红O染色和茜素红染色鉴定。HPMSC经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。根据ScienCell Research Laboratories提供的培养条件，HPMSC可保证进一步扩增15个群体倍增。

推荐培养基

建议使用间充质干细胞培养基（MSCM，产品编号7501）对HPMSC进行体外培养。

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