Telomeres protect our chromosomes and shorten with each cell division, a process tied to aging. Telomerase, the enzyme that replenishes these protective caps, is essential for stem cell function and cancer progression. Measuring telomerase activity is critical for understanding aging, cancer, and stem
In CNS research, understanding disease mechanisms goes beyond tracking individual biomarkers—it requires insight into how entire biological pathways are regulated. From blood–brain barrier (BBB) integrity to glial activation and neuroinflammatory signaling, meaningful studies depend on high-resolution
The 2017 Nobel Prize in Physiology or Medicine was awarded to Jeffrey C Hall, Michael Rosbash, and Michael W Young for research that established key mechanistic principles on how circadian rhythms are regulated. Circadian rhythms are endogenous oscillations adjusted to changing external cues and driven
In this final installment on how to improve your qPCR gene expression profiling (see 6 Tips to Improve qPCR: part 1 and part 2 for previous discussion), we will discuss pipetting tips and the benefits of a separate reverse transcription step in qPCR template preparation.
Tip #5. Good Laboratory Practice
Despite being essential for any experiment involving DNA replication, people rarely give primers a second thought. To coincide with the launch of our new qPCR gene reference tool, we’ll be giving them a little more of the praise they deserve.
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Dye-based qPCR assays, such as SYBR® Green and EvaGreen®, have many advantages over probe-based qPCR assays. Dye-based systems are desirable because they are highly adaptable, cost effective, and time efficient. qPCR specificity, however, is a vital factor to consider when using dye-based qPCR assays.
