When working with primary cells, it is important to remember that they are not cell lines and should be treated with care. At ScienCell, we specialize in primary cell culture and we are very familiar with the common problems researchers encounter when culturing them. We have compiled a list with 13
Primary cells, which are isolated directly from tissue, show normal cell morphology and maintain many of the important markers and functions seen in vivo. Primary cells, though, have a finite lifespan and limited expansion capacity, so it is critical to use low passage primary cells for your research.
Neuronal cell lines are commonly used for in vitro neurobiology studies because they are more easily transfected compared to primary neurons and they proliferate, whereas primary neurons do not. Neuronal cell lines can be induced to differentiate into neuron-like cells, where they express neuronal markers
Cell culturing is a widely used technique to grow cells outside of their natural environment using artificial environments and controlled conditions that has become indispensable to scientific research. The applications of cell culturing are innumerable and there are many ways to differentiate types
When it comes to primary cell culture, a picture is worth a thousand words. A quality image can convey information on cell morphology, health, culture purity and density. Different types of 2-dimensional microscopy techniques such as phase contrast, relief contrast, and fluorescence microcopy can
Many primary cells have difficulty adhering to uncoated glass and plastic surfaces, especially in low serum or serum-free conditions. For primary cells, it is necessary to use a substrate coating to enhance cell attachment. There are many different substrates to choose from and each type can have dramatically
Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical role in scientific advancements, yet researchers have become increasingly cautious when interpreting
When working with primary cells, it is important to remember that they are not cell lines and cannot be treated the same. Because ScienCell has extensively working with primary cells, we are very familiar with the common problems researchers encounter when culturing them. Here are six common mistakes
Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical role in scientific advancements, yet researchers have become increasingly cautious when interpreting
For decades, researchers have been culturing cells to study normal and diseased biological processes. Many of these studies use immortalized cell lines which proliferate indefinitely in culture. Immortalized cells thrive in classical media formulated with the minimum requirements for cell growth, including
