GeneQuery™ 人神经干细胞标志物 qPCR 检测芯片试剂盒

ScienCell's GeneQuery™ Human Neural Stem Cell Markers qPCR Array Kit (GQH-NSC) is designed to facilitate gene expression profiling of 40 marker genes commonly used to assess multipotent neural stem cell maintenance, proliferation, and differentiation.

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

ScienCell 的 GeneQuery™ 人神经干细胞标志物 qPCR 检测芯片试剂盒（GQH-NSC）旨在帮助研究人员分析 40 种常用于评估多潜能神经干细胞维持、增殖及分化状态的标志基因的表达情况。

GeneQuery™ qPCR 检测芯片试剂盒采用 96 孔板形式，开盒即可用于 qPCR 实验。每个孔中均预置一组引物，可特异性识别并高效扩增目标基因的互补 DNA（cDNA）。引物经过精心设计，以确保以下性能：（1）在 qPCR 分析中，最佳退火温度为 65°C（Mg²⁺ 浓度为 2 mM，且不添加 DMSO）；（2）除非另有说明，每组引物均可识别目标基因所有已知的转录本变体；（3）每组引物仅扩增一个目标基因。所有引物组均经过 qPCR 熔解曲线分析和凝胶电泳验证。

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