GeneQuery™ 人神经分化标志物 qPCR 检测芯片试剂盒

ScienCell's GeneQuery™ Human Neural Differentiation Markers qPCR Array Kit (GQH-NDM) is designed to facilitate gene expression profiling of 40 marker genes involved in neural differentiation to specific neural lineages. Brief examples of how included genes may be grouped according to their functions are shown below:     

• Immature neuron markers: DCX, NEUROD1, STMN1, TUBB3
• Mature neuron markers: MAP2, NEFH, NEFM, RBFOX3
• Astrocyte markers: GFAP, GLUL, S100B, SLC1A2, S100A6, NFIX, NFIC
• Schwann cell markers: GAP43, MPZ, NCAM1, S100A1, NGFR, EGR2, POU3F1
• Oligodendrocyte markers: CSPG4, MBP, MOG, OLIG1, OLIG2, PDGFRA, SOX10
• Radial glial cell markers: CDH2, FABP7, GFAP, HES1, HES5, NES, PAX6, VIM
• Neuroepithelial cell markers: CDH1, HES1, NES, NOTCH1, OCLN, SOX2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

ScienCell 的 GeneQuery™ 人神经分化标志物 qPCR 检测芯片试剂盒（GQH-NDM）旨在帮助研究人员分析参与神经细胞向特定细胞谱系分化的 40 种标志基因的表达情况。以下列举了部分试剂盒所包含的基因，并根据其功能进行分类：

• 未成熟神经元标志物： DCX、NEUROD1、STMN1、TUBB3
• 成熟神经元标志物： MAP2、NEFH、NEFM、RBFOX3
• 星形胶质细胞标志物： GFAP、GLUL、S100B、SLC1A2、S100A6、NFIX、NFIC
• 施旺细胞标志物： GAP43、MPZ、NCAM1、S100A1、NGFR、EGR2、POU3F1
• 少突胶质细胞标志物： CSPG4、MBP、MOG、OLIG1、OLIG2、PDGFRA、SOX10
• 放射状胶质细胞标志物： CDH2、FABP7、GFAP、HES1、HES5、NES、PAX6、VIM
• 神经上皮细胞标志物： CDH1、HES1、NES、NOTCH1、OCLN、SOX2

GeneQuery™ qPCR 检测芯片试剂盒采用 96 孔板形式，可直接用于 qPCR 实验。每个孔中均预置一组引物，可特异性识别并高效扩增目标基因的互补 DNA（cDNA）。引物经过精心设计，以确保以下性能：（1）在 qPCR 分析中，最佳退火温度为 65°C（Mg²⁺ 浓度为 2 mM，且不添加 DMSO）；（2）除非另有说明，每组引物均可识别目标基因所有已知的转录本变体；（3）每组引物仅扩增一个目标基因。所有引物组均经过 qPCR 熔解曲线分析和凝胶电泳验证。

Search for a Certificate of Analysis by Lot Number below:

Frequently Asked Questions →

Find answers to common questions about ScienCell products, cell culture, handling, and research applications.

No Publication available at this time.