兔肾近端小管上皮细胞

Renal proximal tubular epithelial cells (RPTEpiC) play a crucial role in renal function. They reabsorb nearly all the glucose and amino acids in the glomerular filtrate, while allowing substances of no nutritional value to be excreted into the urine. RPTEpiC are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. RPTEpiC can produce inflammatory mediators, such as cytokines or chemokines, and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. RPTEpiC express IL-2R alpha and MHC class II antigens during inflammation, after renal transplantation, and during crescentic glomerulonephritis, suggesting that these cells have the capacity to participate in the pathogenesis of immune renal injury. Rabbit RPTEpiC (RabRPTEpiC) are a useful model for studying their role in renal disease and injury.

RabRPTEpiC from ScienCell Research Laboratories are isolated from rabbit kidney. RabRPTEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RabRPTEpiC are characterized by immunofluorescence with antibody specific to cytokeratin-18. RabRPTEpiC are negative for mycoplasma, bacteria, yeast, and fungi. RabRPTEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RabRPTEpiC are not recommended for long-term cultures due to limited expansion capacity and senescence after subculturing.

Recommended Medium

It is recommended to use Epithelial Cell Medium-animal (EpiCM-a, Cat. #4131) for culturing RabRPTEpiC in vitro.

肾近端小管上皮细胞（RPTEpiC）在肾脏功能中发挥关键作用。它们几乎能够重吸收肾小球滤液中的全部葡萄糖和氨基酸，同时允许无营养价值的物质排出至尿液中。RPTEpiC 也是多种先天性、代谢性及炎症性疾病中主要的损伤靶细胞。在炎症过程中，RPTEpiC 可产生多种炎症介质，如细胞因子和趋化因子，并通过分泌 IL-8 影响和引导白细胞趋化，从而积极参与急性炎症反应。在肾移植后及新月体性肾小球肾炎等炎症状态下，RPTEpiC 可表达 IL-2R α 及 MHC II 类抗原，提示其可能参与免疫介导的肾损伤发生机制。兔肾近端小管上皮细胞（RabRPTEpiC）是研究肾脏疾病及损伤机制的有用体外模型。

RabRPTEpiC 由 ScienCell Research Laboratories 提供，分离自兔肾组织。RabRPTEpiC 在第一代（P1）时进行冷冻保存，并以冻存状态交付。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。RabRPTEpiC 通过针对细胞角蛋白-18（cytokeratin-18）的特异性抗体进行免疫荧光鉴定。经检测，RabRPTEpiC 不含支原体、细菌、酵母及真菌污染。RabRPTEpiC 在 ScienCell Research Laboratories 提供的培养条件下可继续培养；但由于其扩增能力有限，并在传代后易出现衰老，因此不推荐长期培养。

推荐培养基

建议使用 Epithelial Cell Medium-animal（EpiCM-a，产品编号 #4131）进行 RabRPTEpiC 的 体外（in vitro） 培养。