CD1来源小鼠脊髓神经元

The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain. Neurons are dynamically polarized cells responsible for electrochemically transmitting information throughout the nervous system. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. The spinal cord is a central nervous system structure connecting the brain and body. It functions as a conduit for sensory information from the extremities and trunk to the brain and transmits voluntary movements from the brain to muscles. Primary mouse neurons-spinal cord (MN-sc) can be used to study the potential treatments for spinal cord injuries.

MN-sc from ScienCell Research Laboratories are isolated from the embryonic day 14 CD-1 mouse spinal cord. MN-sc are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. MN-sc are characterized by immunofluorescence with antibodies specific to β-tubulin III. MN-sc are negative for mycoplasma, bacteria, yeast, and fungi. MN-sc are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MN-sc are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.

Recommended Medium
It is recommended to use neuronal medium (NM, Cat. #1521) for culturing MN-sc in vitro.

中枢神经系统组织主要由两大类细胞组成，即神经元和胶质细胞。神经元是大脑的解剖、功能和营养支持基本单位。神经元具有动态极性，能够通过电化学信号在整个神经系统中传递信息。尽管神经元在大小和形态上存在显著差异，但它们均具有共同的形态学特征，是构成高度复杂信息交流网络的基本要素。脊髓是连接大脑与身体的中枢神经系统结构，负责将来自四肢和躯干的感觉信息传递至大脑，并将大脑发出的随意运动指令传递至肌肉。原代小鼠脊髓神经元（MN-sc）可用于研究脊髓损伤的潜在治疗方法。

ScienCell Research Laboratories提供的小鼠脊髓神经元（MN-sc）分离自胚胎第14天（E14）的CD-1小鼠脊髓。MN-sc在第0代（P0）时进行冻存，并以冷冻状态运输。每瓶含有超过1 × 10⁶个细胞，体积为1 ml。MN-sc采用针对β-III型微管蛋白（β-tubulin III）的特异性抗体进行免疫荧光鉴定。经检测，MN-sc不含支原体、细菌、酵母和真菌。按照ScienCell Research Laboratories提供的培养条件，MN-sc可继续进行体外培养；但是，由于MN-sc在培养过程中不会增殖，因此不建议用于细胞扩增或长期培养。

推荐培养基
推荐使用神经元培养基（NM，产品编号1521）进行MN-sc的体外培养。

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