Lactate is an important intermediary in glucose metabolism. Under hypoxic or anaerobic conditions, lactate dehydrogenase (LDH) converts pyruvate, the final product of glycolysis, to lactate. Monitoring lactate levels is an adequate indicator of the balance between tissue oxygen demand and utilization, and is useful when studying cellular physiology. This colorimetric assay is based on the reduction of the tetrazolium salt, INT, in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm. The intensity of the optical density is proportional to the lactate concentration.
乳酸是葡萄糖代谢过程中的重要中间产物。在缺氧或无氧条件下，乳酸脱氢酶（LDH）可将糖酵解终产物丙酮酸转化为乳酸。监测乳酸水平能够有效反映组织氧供与氧利用之间的平衡状态，是研究细胞生理功能和代谢状态的重要指标。本比色法检测基于 NADH 偶联酶促反应，四唑盐 INT 被还原生成甲臜（Formazan）产物，其在 490 nm 波长处具有最大吸光度。测得的光密度（OD）值与样品中的乳酸浓度成正比。
| 目录编号 | 8308 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | LAC |
| 规格/数量 | 100 Tests in 96-well plate |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请将 Assay Buffer（#8308a）和 Reconstitution Solution（#8308e）存放于 4°C；L-乳酸标准品（#8308b）、酶混合物（#8308c）和底物混合物（#8308d）存放于 -20°C。 |
| 运输 | 干冰。 |