人多能干细胞来源的皮层神经元

Cortical neurons are the principal excitatory projection neurons of the cerebral cortex and are central to information processing across sensory perception, cognition, and voluntary motor control through organized laminar microcircuits and long-range connectivity. During development and early life, cortical circuits undergo experience-dependent refinement (“critical period” plasticity), a process that strongly shapes synaptic connectivity and functional network maturation. Disruption of cortical development or circuit function has been implicated in numerous neurological and neuropsychiatric disorders, and in vitro human cortical neuron models provide high value for mechanistic discovery and drug screening. Human PSC-based corticogenesis platforms have demonstrated robust generation of cortical projection neuron subtypes in a temporal sequence and the formation of functional excitatory synapses and networks, supporting their use as scalable, standardized human model systems. Human Pluripotent Stem Cell-derived Cortical Neurons (HPSC-CN) from ScienCell Research Laboratories provide a robust, reliable, and consistent off-the-shelf option for human cortical neurons. 

HPSC-CN are cryopreserved and delivered frozen. Each vial contains > 1 × 10⁶ cells in 1 ml volume. HPSC-CN are characterized by immunofluorescence with antibodies specific to pan neuronal marker beta tubulin III (Tubb3) and cortical identity marker TBR1. HPSC-CN are negative for mycoplasma, bacteria, yeast, and fungi. HPSC-CN can be further cultured under the conditions provided by ScienCell Research Laboratories. HPSC-CN are not recommended for expansion since the cells do not proliferate in culture. 

Recommended Medium 
It is recommended to use Neuronal Maintenance Medium (NMM, Cat. #5951) for culturing HPSC-CN in vitro.