人源中耳成纤维细胞

The middle ear is composed of three auditory ossicles (incus, malleus, and stapes), the tympanic membrane, and the Eustachian tube. The middle ear functions to transfer acoustic energy from the tympanic membrane to the cochlea. Middle Ear Fibroblasts (MEF), which are located in the middle ear, are mesenchymal cells derived from the embryonic mesoderm. In general, fibroblasts are responsible for extracellular matrix synthesis in connective tissue and play major roles in wound healing. Studies indicate that human MEF may also contribute to pathogenesis of cholesteatoma, or abnormal skin growth in the middle ear, by producing inflammatory factors that result in epithelial cell proliferation. Human MEF (HMEF) are a useful model to elucidate the role of  fibroblasts in the development of middle ear cholesteatoma.

HMEF from ScienCell Research Laboratories are isolated from human middle ear. HMEF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HMEF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HMEF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMEF are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HMEF in vitro.

中耳由三块听小骨（砧骨、锤骨和镫骨）、鼓膜以及咽鼓管组成。中耳的功能是将声能从鼓膜传递至耳蜗。位于中耳内的中耳成纤维细胞（MEF）是来源于胚胎中胚层的间充质细胞。通常情况下，成纤维细胞负责结缔组织中细胞外基质的合成，并在伤口愈合过程中发挥重要作用。研究表明，人源 MEF 可能通过产生促进上皮细胞增殖的炎症因子，参与胆脂瘤的发病过程，即中耳内异常皮肤样组织的增生。人源中耳成纤维细胞（HMEF）是一种有用的研究模型，可用于阐明成纤维细胞在中耳胆脂瘤形成过程中的作用。

ScienCell Research Laboratories 提供的人源中耳成纤维细胞（HMEF）分离自人源中耳组织。HMEF 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HMEF 根据其梭形细胞形态以及采用针对纤连蛋白的特异性抗体进行免疫荧光鉴定。HMEF 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HMEF 可保证进一步扩增 10 个群体倍增周期。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 #2301）对 HMEF 进行体外培养。