人源中耳上皮细胞

The middle ear is composed of three auditory ossicles (incus, malleus, and stapes), the tympanic membrane, and the Eustachian tube. The middle ear functions to transfer acoustic energy from the tympanic membrane to the cochlea. Middle Ear Epithelial Cells (MEEpiC) play an important role in homeostasis and sterility of the middle ear. Abnormalities associated with MEEpiC can lead to the development of middle ear disorders such as otitis media and cholesteatoma. Evidence indicates that epithelial cell proliferation is the underlying cause of cholesteatoma. Recent studies also suggest that the abnormal epithelial cell proliferation may be linked to inflammatory factors produced by middle ear fibroblasts. Human MEEpiC (HMEEpiC) are a useful in vitro model for studying middle ear diseases and may be used to identify new therapeutic targets for otitis media and cholesteatoma.

HMEEpiC from ScienCell Research Laboratories are isolated from human middle ear. HMEEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HMEEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HMEEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMEEpiC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Epithelial Cell Medium-2 (EpiCM-2 #4121) for culturing HMEEpiC in vitro.

中耳由三块听小骨（砧骨、锤骨和镫骨）、鼓膜以及咽鼓管组成。中耳的功能是将声能从鼓膜传递至耳蜗。中耳上皮细胞（MEEpiC）在维持中耳稳态和无菌环境方面发挥重要作用。与 MEEpiC 相关的异常可导致中耳疾病的发生，例如中耳炎和胆脂瘤。研究证据表明，上皮细胞增殖是胆脂瘤形成的根本原因。近期研究还表明，异常的上皮细胞增殖可能与中耳成纤维细胞产生的炎症因子有关。人源中耳上皮细胞（HMEEpiC）是研究中耳疾病的一种有用的体外模型，可用于发现治疗中耳炎和胆脂瘤的新型治疗靶点。

ScienCell Research Laboratories 提供的人源中耳上皮细胞（HMEEpiC）分离自人源中耳组织。HMEEpiC 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有 超过 5 x 10⁵ 个细胞，体积为 1 ml。HMEEpiC 经采用针对细胞角蛋白-18 和/或细胞角蛋白-19 的特异性抗体进行免疫荧光鉴定。HMEEpiC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HMEEpiC 可保证进一步扩增 15 个群体倍增周期。

推荐培养基

建议使用上皮细胞培养基-2（EpiCM-2 #4121）对 HMEEpiC 进行体外培养。