GeneQuery™ HLA-A*02 qPCR 分型检测试剂盒

The human leukocyte antigen (HLA) complex is a gene complex located on the short arm of human chromosome 6. The human version of the major histocompatibility complex (MHC) helps the immune system distinguish the host cells from invaders. It is the most polymorphic locus in the human genome, and has been implicated in many pathologies including autoimmunity disorders and cancer.

GeneQuery™ HLA-A*02 Screening Kit by qPCR (GSHA02) utilizes SYBR®Green-based qPCR with melt curve analysis to reveal the presence or absence of HLA-A*02 allele in a human cell sample. GSHA02 can utilize either cell lysate or purified genomic DNA as the qPCR template. The cell lysis buffer and enhancer (Cat #GQ400a and GQ400b) are included in the kit to lyse pelleted cell samples. Each GSHA02 kit contains a HLA-A*02 (A02) primer set and a genomic DNA Control (GDC) primer set. The A02 primer set amplifies only the HLA-A*02 allele, but not other HLA-A alleles. The GDC primer set targets a region on human chromosome 21 and is included to examine the quality of the test sample, qPCR components, and qPCR conditions. Each primer set included in GTM-HA26 has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity. The 2X GoldNStart TaqGreen qPCR Master Mix (Cat #MB6018a-1) is a SYBR®Green dye-based qPCR master mix with a “hot-start” property. It contains SYBR®Green, dNTPs, Taq DNA polymerase, and an inert gold-color loading indicator in a single tube. The “hot-start” property achieved through ScienCell’s unique chemically modified Taq DNA polymerase provides maximal inhibition of primer dimer formation. The advanced buffer formulation provides superior specificity and efficiency with a wide linear dynamic range. The inert gold-color loading indicator allows for better visualization and tracking of sample loading in qPCR plates or tubes. The HLA-A*02 positive genomic DNA control (Cat #GK830b) is the gDNA extracted from HLA-A*02 positive cells. The kit provides enough reagents for running 10 qPCR reactions each with the A02 and the GDC primer sets.

Additional Materials Required (Materials Not Included in Kit)

人类白细胞抗原（Human Leukocyte Antigen，HLA）复合体位于人类 6 号染色体短臂，是人类主要组织相容性复合体（Major Histocompatibility Complex，MHC）的组成部分，可帮助免疫系统识别自身细胞与外来入侵物。HLA 是人类基因组中多态性最高的基因区域之一，与多种疾病密切相关，包括自身免疫性疾病和肿瘤等。

GeneQuery™ HLA-A*02 qPCR 基因分型筛查试剂盒（GSHA02）采用基于 SYBR® Green 的实时荧光定量 PCR（qPCR）结合熔解曲线分析技术，可检测人细胞样本中是否携带 HLA-A*02 等位基因。GSHA02 可直接使用细胞裂解液或纯化的基因组 DNA 作为 qPCR 扩增模板。试剂盒内含细胞裂解缓冲液及其增强剂（产品编号：GQ400a 和 GQ400b），可用于裂解离心收集的细胞沉淀。每套 GSHA02 均配备 HLA-A*02（A02）特异性引物组和基因组 DNA 对照（GDC）引物组。其中，A02 引物组仅扩增 HLA-A*02 等位基因，而不会扩增其他 HLA-A 等位基因；GDC 引物组靶向人 21 号染色体特定区域，用于评估检测样本质量、qPCR 试剂性能及反应条件是否正常。所有引物组均已通过 qPCR 熔解曲线分析和凝胶电泳验证，具有良好的扩增特异性。

试剂盒配套的 2× GoldNStart TaqGreen qPCR 预混液（产品编号：MB6018a-1）是一款基于 SYBR® Green 染料的热启动型（Hot-Start）qPCR 预混液，单管中包含 SYBR® Green、dNTP、Taq DNA 聚合酶及惰性金色上样指示剂。其热启动特性基于 ScienCell 独有的化学修饰 Taq DNA 聚合酶技术，可最大程度抑制引物二聚体形成。优化的缓冲体系可提供优异的扩增特异性和扩增效率，并具有较宽的线性动态检测范围。惰性金色上样指示剂有助于在 qPCR 板或 PCR 管中直观观察样品加样情况，提高实验操作的准确性。

HLA-A*02 阳性基因组 DNA 对照（产品编号：GK830b）来源于 HLA-A*02 阳性细胞提取的基因组 DNA。本试剂盒提供的试剂可分别使用 A02 引物组和 GDC 引物组各完成 10 次 qPCR 检测反应。

所需其他材料（试剂盒未提供）