GeneQuery™ 人癌症干细胞标志物 qPCR 检测芯片试剂盒

ScienCell's GeneQuery™ Human Cancer Stem Cell Markers qPCR Array Kit (GQH-CSC) is designed to facilitate gene expression profiling of 40 commonly used cancer stem cell markers. Cancer stem cells (CSCs), also known as cancer initiating cells, are a small subpopulation of immortal cells within a tumor or hematological cancers that are capable of self-renewal and differentiation. CSCs have been linked to cancer initiation, progression and metastasis and may serve as targets for cancer treatment.

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

ScienCell 的 GeneQuery™ 人癌症干细胞标志物 qPCR 检测芯片试剂盒（GQH-CSC）旨在帮助研究人员分析 40 种常用癌症干细胞标志物的基因表达情况。癌症干细胞（CSCs），又称肿瘤起始细胞，是存在于实体瘤或血液系统恶性肿瘤中的一小群具有自我更新和分化能力的细胞。癌症干细胞与肿瘤的发生、进展及转移密切相关，因此可能成为癌症治疗的重要靶点。

GeneQuery™ qPCR 检测芯片试剂盒采用 96 孔板形式，可直接用于 qPCR 实验。每个孔中均预置一组引物，可特异性识别并高效扩增目标基因的互补 DNA（cDNA）。引物经过精心设计，以确保以下性能：（1）在 qPCR 分析中，最佳退火温度为 65°C（Mg²⁺ 浓度为 2 mM，且不添加 DMSO）；（2）除非另有说明，每组引物均可识别目标基因所有已知的转录本变体；（3）每组引物仅扩增一个目标基因。所有引物组均经过 qPCR 熔解曲线分析和凝胶电泳验证。

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