Epithelial Cell Medium

Epithelial Cell Medium (EpiCM), when used with Epithelial Cell Growth Supplement (EpiCGS, Cat #4152) and 10 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO₂ /95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal epithelial cells in vitro.