DMEM/F-12 培养基（含 L-谷氨酰胺和 HEPES）

During the past two decades, researchers have been seeking serum-free media for the culturing of a wide range of cell lines and even primary cells. With the supplementation of growth factors and hormones, researchers discovered that a 1:1 mixture of DMEM and Ham's F-12 Nutrient Mixture was beneficial to a wide ranges of cells. HEPES buffer is included in the formulation at a final concentration of 15 mM to compensate for the loss of buffering capacity incurred by eliminating serum. DMEM/F12 with L-glutamine and HEPES is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. It is well suited for supporting the growth of a broad range of mammalian cell lines.

过去二十年来，研究人员一直致力于开发适用于多种细胞系甚至原代细胞培养的无血清培养基。通过补充生长因子和激素，研究发现 DMEM 与 Ham's F-12 营养混合液按 1:1 比例混合的培养基能够为多种细胞提供良好的生长支持。为弥补去除血清后缓冲能力的降低，该配方中添加了终浓度为 15 mM 的 HEPES 缓冲剂，以增强培养基的 pH 稳定性。

DMEM/F-12 培养基（含 L-谷氨酰胺和 HEPES）是一种无菌液体培养基，包含必需氨基酸和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子以及微量矿物质等成分，适用于支持多种哺乳动物细胞系的生长。