过氧化氢酶（CAT）活性检测试剂盒

Catalase is an antioxidant enzyme present in most living organisms which are exposed to oxygen. It is involved in the decomposition of hydrogen peroxide (H2O2), a reactive oxygen species (ROS) which is a toxic product of normal aerobic metabolism. ScienCell Catalase Activity (CAT) Assay measures the activity of catalase based on the peroxidatic function of catalase with methanol as the hydrogen donor in the presence of H2O2. The formaldehyde produced is determined with purpald (4-Amino-3-hydrazino-5-mercapto-1,2,4-triazole) as a chromogen. To obtain a colored compound, the product of the reaction between formaldehyde and purpald is oxidized by potassium periodate. The absorbance can be read at 550 nm.

过氧化氢酶（Catalase, CAT）是一种广泛存在于需氧生物体内的抗氧化酶，参与分解过氧化氢（H₂O₂）。过氧化氢是一种活性氧（ROS），是正常有氧代谢过程中产生的具有毒性的副产物。

ScienCell 过氧化氢酶活性（CAT）检测试剂盒基于CAT的过氧化物酶活性原理，在H₂O₂存在条件下，以甲醇作为氢供体进行反应。反应生成的甲醛通过显色剂Purpald（4-氨基-3-肼基-5-巯基-1,2,4-三唑）进行检测。

为形成有色化合物，甲醛与Purpald反应生成的产物进一步在高碘酸钾的作用下被氧化。最终产物的吸光度可在550 nm处测定。