页面 2 - sciencell blog

Recently, bone marrow mononuclear cells (BMMC) have been in the spotlight for having the potential to treat a variety of diseases including neurological disorders. Why are BMMC so exciting and do they really show treatment potential? BMMC have regenerative capabilities and are a heterogeneous population

Despite being essential for any experiment involving DNA replication, people rarely give primers a second thought.  To coincide with the launch of our new qPCR gene reference tool, we’ll be giving them a little more of the praise they deserve.

In this next installment on how to improve your qPCR gene profiling (see 6 Tips to Improve qPCR for previous discussion), we would like to discuss the limitations of melting curve analysis and PCR inhibitors.

Tip #3. Melting curve analysis is NOT sufficient for assessing qPCR product specificity.

In

Gene expression analysis is critical for understanding the transcriptome profiles of primary cells and how they directly influence the cells’ functionality. In addition, the transcriptome profile can affect cell proliferation, differentiation, senescence, or apoptosis. Traditional reporter-gene assays

Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical role in scientific advancements, yet researchers have become increasingly cautious when interpreting