pcr
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发布: 十二月 19, 2024完整阅读 »
What is DNA/RNA Isolation?
DNA/RNA isolation, also referred as nucleic acid purification, is the process of extracting and purifying DNA or RNA from biological samples like cells, tissues, and body fluids.
The purpose of DNA/RNA isolation is to obtain pure nucleic acid samples free from contaminants
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发布: 可能 03, 2017完整阅读 »
3 Reasons Why You Should Take "Pseudogenes" Seriously in Your Research
Protein-coding DNA, or “functional” genes, only accounts for up to 2% in the human genome. Until recently, the remaining 98% have been referred to as “junk DNA” for their putative noncoding feature. The name itself implies that junk
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发布: 八月 23, 2016完整阅读 »
In this final installment on how to improve your qPCR gene expression profiling (see 6 Tips to Improve qPCR: part 1 and part 2 for previous discussion), we will discuss pipetting tips and the benefits of a separate reverse transcription step in qPCR template preparation.
Tip #5. Good Laboratory Practice
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完整阅读 »
Despite being essential for any experiment involving DNA replication, people rarely give primers a second thought. To coincide with the launch of our new qPCR gene reference tool, we’ll be giving them a little more of the praise they deserve.
Dr Kary Mullis received the 1993 Nobel Prize in Chemistry -
发布: 二月 03, 2016完整阅读 »
Gene expression analysis is critical for understanding the transcriptome profiles of primary cells and how they directly influence the cells’ functionality. In addition, the transcriptome profile can affect cell proliferation, differentiation, senescence, or apoptosis. Traditional reporter-gene assays