eNOS, Nitric Oxide Signaling, and Endothelial Function
Endothelial nitric oxide synthase (eNOS), encoded by NOS3, is the primary source of nitric oxide (NO) in the vascular endothelium. NO produced by eNOS regulates vascular tone, endothelial permeability, platelet activation, leukocyte adhesion, angiogenesis, and the inflammatory state of the vessel wall.
Impaired NO bioavailability is a defining feature of endothelial dysfunction and has been associated with atherosclerosis, hypertension, coronary artery disease, metabolic disease, and vascular aging. Because eNOS regulates multiple endothelial functions, NOS3 genotype can be relevant to a broad range of vascular biology assays — including angiogenesis, migration, permeability, oxidative stress, and inflammatory signaling.
Neither variant alone determines cardiovascular disease outcome. Effects are probabilistic and interact with other genetic and environmental factors.
Confirmed Genotypes in Aortic and Coronary Artery Endothelial Cells
| Cell Type | Catalog No. | rs1799983 (Glu298Asp) | rs2070744 (-786T>C) |
|---|---|---|---|
| Human Aortic Endothelial Cells | Cat. #6100 | GG (WT) TT (Hom Var) | TT (WT) CT (Het) CC (Hom Var) |
| Human Coronary Artery Endothelial Cells | Cat. #6020 | GG (WT) GT (Het) TT (Hom Var) | TT (WT) CT (Het) |
Control NOS3 Genotype as an Experimental Variable
Primary endothelial cells from donors of unknown NOS3 genotype carry uncharacterized variation at these loci — variation that influences baseline eNOS expression, NO production, and responses to experimental stimuli. Immortalized endothelial lines offer reproducibility but may not faithfully reflect primary human eNOS biology. iPSC-derived and CRISPR-edited models allow isolation of individual variants but lack the broader native donor genetic background.
Control a Key Biological Variable
Select donor APOE genotype as part of experimental design rather than treating genotype as background variability.
Compare Defined Genetic Backgrounds
Run APOE3/3 and APOE4/4 astrocytes side-by-side under identical culture conditions in the same experiment.
Study Genotype-Dependent Responses
Characterize how APOE genotype influences lipid handling, inflammatory signaling, and drug response in primary human astrocytes.
Preserve Native Donor Biology
Primary astrocytes retain donor-specific cellular characteristics and native human signaling responses that can complement developmentally derived iPSC-astrocyte models.
Complement Existing Models
Use genotype-defined primary astrocytes alongside iPSC-derived or CRISPR-edited astrocytes to build a more complete picture of APOE-dependent mechanisms.
Pharmacogenomic Screening
Screen compounds against APOE3/3 and APOE4/4 backgrounds to identify genotype-dependent therapeutic responses before advancing to in vivo models.
Applications Specific to NOS3 Endothelial Biology
Products
Human Aortic Endothelial Cells — Cat. #6100
Confirmed genotypes at rs1799983 (GG, TT) and rs2070744 (TT, CT, CC). Recommended medium: Endothelial Cell Medium (ECM, Cat. #1001).
View product →Human Coronary Artery Endothelial Cells — Cat. #6020
Confirmed genotypes at rs1799983 (GG, GT, TT) and rs2070744 (TT, CT). Recommended medium: Endothelial Cell Medium (ECM, Cat. #1001).
View product →Requesting NOS3 Genotype-Defined Lots
Genotype-defined lots are available by request. Each lot ships with a COA confirming genotype at both NOS3 loci. A selection and verification fee applies per vial.
Identify the Cell Type
Identify the endothelial cell type — aortic (Cat. #6100) or coronary artery (Cat. #6020)
Specify NOS3 Genotype
Specify the NOS3 variant(s) and genotype(s) required — coding variant (rs1799983), promoter variant (rs2070744), or both.
Submit Your Request
Our team will confirm available lots and recommend the right genotype configuration for your study design
Please note that availability varies by genotype and donor lot. A selection and verification fee will be applied per vial and reflected in your quote.
For information on available genotypes, assistance selecting the right cells, or inquiries about additional genotypes and cell types, please contact us at info@sciencellonline.com, or call 1.877.602.8549.