Human Umbilical Artery Endothelial Cells
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Isolated from human umbilical arteries. HUAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume.
Vascular endothelial cells contribute to the maintenance of vascular homeostasis. Vascular endothelial cells produce and secrete activators and inhibitors of the coagulation and fibrinolysis system. In addition, they mediate the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that regulate cell proliferation and control vessel wall tone. Human umbilical vein endothelial cells (HUVEC) and human umbilical artery endothelial cells (HUAEC) are the most commonly used cell type for the study of endothelial cell processes in vitro. HUAEC have a “cobblestone” morphology, show positive staining for vWF/Factor VIII and CD-31, and the ability to take up acetylated low-density lipoprotein [1, 2]. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin [3] and VCAM [4].
HUAEC from ScienCell Research Laboratories are isolated from human umbilical arteries. HUAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HUAEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM) and by uptake of DiI-Ac-LDL. HUAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUAEC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HUAEC in vitro.
| Catalog No. | 8010 |
|---|---|
| Country of Manufacture | United States |
| Promotion Text | Buy One, Get One 50% OFF |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Size/Quantity | 5 x 10^5 cells/vial |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping | Dry ice. |
| Product Code | HUAEC |
| References | [1] Morgan, D. M. L. (1996) Isolation and culture of human umbilical vein endothelial cells. In Human Cell Culture Protocols (Gareth E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109. [2] Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247:1219-1222. [3] Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243:1160-1165. [4] Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial protein that binds to lymphocytes. Cell 59:1203-1211. |
2.) Shahani T, Lavend'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. (2010) "Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool." Blood. 115: 4902-9.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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