Human Umbilical Artery Endothelial Cells

Vascular endothelial cells contribute to the maintenance of vascular homeostasis. Vascular endothelial cells produce and secrete activators and inhibitors of the coagulation and fibrinolysis system. In addition, they mediate the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that regulate cell proliferation and control vessel wall tone. Human umbilical vein endothelial cells (HUVEC) and human umbilical artery endothelial cells (HUAEC) are the most commonly used cell type for the study of endothelial cell processes in vitro. HUAEC have a “cobblestone” morphology, show positive staining for vWF/Factor VIII and CD-31, and the ability to take up acetylated low-density lipoprotein [1, 2]. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin [3] and VCAM [4].

HUAEC from ScienCell Research Laboratories are isolated from human umbilical arteries. HUAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HUAEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM) and by uptake of DiI-Ac-LDL. HUAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUAEC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HUAEC in vitro.

Product Sheets

8010