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Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. PPase catalyzed reaction is Mg²⁺-dependent. PPase is used for in vitro transcription (IVT) to increase RNA synthesis yield by removing the inhibitory PPi.

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. PPase catalyzed reaction is Mg²⁺-dependent. PPase is used for in vitro transcription (IVT) to increase RNA synthesis yield by removing the inhibitory PPi. Yeast Inorganic Pyrophosphatase is heat-sensitive and loses activity rapidly above 55 °C, avoid prolonged exposure to elevated temperatures.

SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, UBI-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease.

Unit Definition:

One unit of SUMO protease is defined as the amount of enzyme needed to cleave 90% of 100 µg of control SUMO tagged protein at 30°C for 1 hour.

TEV (Tobacco Etch Virus) Protease is a highly specific cysteine protease that recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S) and cleaves between Q and G/S.